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Skip intermediate "sai" files via use of implicit pipes when doing paired-end alignment (Linux/bash)
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#!sh | |
#Must be executed with bash shell. | |
#If sh bwapetrick1.sh fails, then say bash bwapetrick1.sh | |
#bwa 0.7.5a-r405 | |
#samtools 0.1.19 | |
LEFTREADS=NY42_06_21_2010_54_1.fastq.gz | |
RIGHTREADS=NY42_06_21_2010_54_2.fastq.gz | |
REFERENCE=dyak-all-chromosome-r1.3-newnames.fasta | |
BAMFILEBASE=lane0 | |
#Bam file creation in 1 stop via named pipes | |
bwa sampe $REFERENCE <(bwa aln -t 16 $REFERENCE $LEFTREADS) <(bwa aln -t 16 $REFERENCE $RIGHTREADS) $LEFTREADS $RIGHTREADS | samtools view -bS - | samtools sort -m 50000000 - $BAMFILEBASE |
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