msuprun

# using Merge 
lists <- list(eset,eset3)
eset_both <- inSilicoMerging::merge(lists, method='NONE')
 
# using combine 
eset_both2 <- combine(eset, eset3)
 
# Manually
neweset<-ExpressionSet(assayData=cbind(exprs(eset),exprs(eset3)[featureNames(eset),]))
phenoData(neweset)<-new("AnnotatedDataFrame",rbind(pData(eset),pData(eset3))[sampleNames(neweset),])

msuprun

Python 

Hamlet example
data here: http://www.gutenberg.org/cache/epub/2265/pg2265.txt

def read_Hamlet():
    my_file = open("hamlet.txt")
    return my_file

my_file = read_Hamlet()