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mutterer/excessive_green_macro.ijm

Created May 7, 2015 10:44
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excessive_green_macro.ijm
run("RGB Stack"); // convert RGB image in a stack
run("32-bit"); // make it 32 bit for computations
run("Multiply...", "value=-1 stack"); // invert values for r,g,b
setSlice(2); // go to the green channel
run("Multiply...", "value=-2 slice"); // -g*-2 = +2g
run("Z Project...", "start=1 stop=3 projection=[Sum Slices]"); // sum all channels result= -r + 2g -b
setAutoThreshold("Intermodes dark"); // auto thresholding now works
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mutterer commented May 7, 2015

This ImageJ macro helps with segmenting image areas that are greener.
I first learned about this method at the ImageJ conference 2010 from Gerrit Polder.

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