Skip to content

Instantly share code, notes, and snippets.

@paolorechia

paolorechia/result_with_events.json

Last active January 23, 2024 21:47
Show Gist options
  • Save paolorechia/f2f9c546fb7eba7022a5cf591815ae8b to your computer and use it in GitHub Desktop.
Save paolorechia/f2f9c546fb7eba7022a5cf591815ae8b to your computer and use it in GitHub Desktop.
Download ZIP
Query Trace with Result Events
Raw
result_with_events.json
{
"name": "chroma.add",
"context": {
"trace_id": "0x07962ec8f4039af52d06588ad041d7c1",
"span_id": "0xaaabab279608b7f0",
"trace_state": "[]"
},
"kind": "SpanKind.INTERNAL",
"parent_id": null,
"start_time": "2024-01-23T21:47:08.733265Z",
"end_time": "2024-01-23T21:47:09.039073Z",
"status": {
"status_code": "UNSET"
},
"attributes": {
"db.system": "chroma",
"db.operation": "add",
"db.chroma.add.ids_count": 10,
"db.chroma.add.metadatas_count": 10,
"db.chroma.add.documents_count": 10
},
"events": [],
"links": [],
"resource": {
"attributes": {},
"schema_url": ""
}
}
{
"name": "chroma.query.segment._query",
"context": {
"trace_id": "0xf348efdbe785092acf97a0253dded059",
"span_id": "0x8b89fa2d16fbf18d",
"trace_state": "[]"
},
"kind": "SpanKind.INTERNAL",
"parent_id": "0xed5d9474bafbc7d3",
"start_time": "2024-01-23T21:47:09.046202Z",
"end_time": "2024-01-23T21:47:09.047445Z",
"status": {
"status_code": "UNSET"
},
"attributes": {
"traceloop.workflow.name": "assess_claims",
"db.system": "chroma",
"db.operation": "_query",
"db.chroma.query.segment._query.collection_id": "d983d28a-8f40-45e0-abed-0f8b84119dd6"
},
"events": [
{
"name": "db.chroma.query.segment._query.query_embeddings.0",
"timestamp": "2024-01-23T21:47:09.046381Z",
"attributes": {
"embeddings": "[-0.07656381279230118, 0.030130451545119286, 0.02478151023387909, -0.01896531693637371, -0.021260280162096024, -0.030420852825045586, -0.022514713928103447, 0.07737720012664795, -0.01356111653149128, 0.021854804828763008, 0.0046875979751348495, -0.01464152429252863, -0.03391741216182709, 0.053753141313791275, -0.03208160400390625, 0.032443128526210785, -0.10043547302484512, 0.001819036086089909, 0.02710125967860222, 0.0796673446893692, -0.04761017486453056, 0.012334557250142097, 0.07045114785432816, -0.005440769251435995, -0.015557944774627686, 0.08534330129623413, 0.06140578165650368, -0.03797881677746773, -0.013787311501801014, -0.038877423852682114, 0.04734493046998978, -0.03659190237522125, -0.058674152940511703, -0.04815930500626564, 0.033464737236499786, -0.05868344381451607, 0.06199783831834793, 0.04070103541016579, -0.03589259460568428, 0.04768437147140503, 0.07261378318071365, 0.004467217717319727, 0.044713061302900314, -0.007377506233751774, 0.09161150455474854, -0.05144480988383293, 0.010295210406184196, -0.08752048015594482, -0.01318000815808773, -0.09739294648170471, 0.038951575756073, 0.014202425256371498, -0.017565639689564705, 0.05457627773284912, 0.0066769360564649105, 0.012620421126484871, -0.014108307659626007, 0.0319986417889595, -0.0014760263729840517, -0.04179004579782486, 0.09589722007513046, -0.050558142364025116, 0.002211816143244505, -0.005073738284409046, 0.017210913822054863, 0.08534817397594452, -0.02914390340447426, 0.0038833857979625463, -0.09331731498241425, 0.017002183943986893, 0.034777067601680756, -0.0015174332074820995, 0.0030266332905739546, 0.05844282731413841, 0.09958583116531372, 0.014838305301964283, -0.004409900866448879, 0.11377041041851044, 0.04079897329211235, 0.1185968890786171, -0.10069656372070312, -0.06240065395832062, -0.014374764636158943, -0.043110162019729614, -0.053355999290943146, 0.10281984508037567, 0.051787760108709335, -0.008153838105499744, -0.10824025422334671, -0.00581357441842556, 0.006937429308891296, 0.06542255729436874, -0.10815658420324326, -0.08664706349372864, -0.04154912754893303, -0.03149183467030525, -0.05570051819086075, -0.002441653748974204, 0.02880827523767948, -0.07149173319339752, -0.030944060534238815, -0.05603637546300888, 0.015968069434165955, 0.07190354913473129, -0.00943480059504509, -0.005982980597764254, 0.021858688443899155, 0.01132160983979702, 0.046600185334682465, 0.03346617892384529, 0.048352017998695374, -0.0013483240036293864, -0.02542315609753132, 0.0626169964671135, -0.08812357485294342, -0.018820395693182945, -0.00542142428457737, 0.023606324568390846, 0.023023517802357674, -0.05859694257378578, 0.07292326539754868, -0.0008137458935379982, -0.0408237986266613, 0.03511868044734001, -0.06876211613416672, -0.0006969192763790488, 0.022884881123900414, -2.2306586714096746e-33, 0.02500869706273079, 0.05055480822920799, 0.050004083663225174, 0.01103924959897995, -0.02038034051656723, -0.06886006146669388, 0.02698659896850586, -0.034593962132930756, 0.07096464931964874, 0.07330244779586792, -0.09925710409879684, 0.11818036437034607, -0.02155531942844391, 0.0700385570526123, -0.00746366661041975, 0.04034028202295303, -0.01555264089256525, -0.02610667422413826, 0.02940523996949196, -0.025897227227687836, 0.04279118403792381, -0.0032851374708116055, 0.012246431782841682, 0.08524347841739655, 0.03546116128563881, -0.11626303195953369, -0.041003305464982986, -0.09017746895551682, -0.07343719899654388, -0.021597201004624367, -0.007728471886366606, 0.03413572907447815, 0.04575314000248909, 0.018161790445446968, -0.008667010813951492, 0.0024382425472140312, -0.0833309218287468, 0.048145610839128494, 0.08865007758140564, 0.030792152509093285, -0.02498656138777733, -0.033218126744031906, 0.0760088637471199, -0.011882591992616653, 0.016330555081367493, -0.014313461259007454, 0.0337657667696476, 0.01145346648991108, -0.013368342071771622, -0.052388258278369904, 0.009955596178770065, 0.025716952979564667, -0.0036768775898963213, -0.028610073029994965, 0.03213721886277199, 0.05366727337241173, -0.01698038913309574, 0.009991336613893509, -0.059916336089372635, -0.06256769597530365, -0.010681577026844025, 0.03530453145503998, 0.007038889452815056, -0.011067286133766174, -0.02855578064918518, 0.00204453244805336, -0.0014927450101822615, 0.00637526111677289, 0.020923055708408356, -0.017787467688322067, 0.02528119646012783, 0.06479019671678543, -0.06370831280946732, -0.06497768312692642, 0.07887062430381775, -0.002710864879190922, 0.03972998633980751, -0.0647626742720604, 0.031816884875297546, 0.08506196737289429, 0.018578726798295975, 0.06295695155858994, -0.10136693716049194, -0.06991083174943924, -0.0371888168156147, -0.045910440385341644, 0.032939814031124115, -0.006651212926954031, -0.023204615339636803, -0.008005320094525814, 0.05859941616654396, -0.012680456042289734, 0.04815012961626053, -0.0032374360598623753, 0.00039943624869920313, -7.023474053697548e-34, 0.021053925156593323, -0.11156169325113297, 0.0760699212551117, 0.008595971390604973, 0.020779963582754135, 0.05312465876340866, 0.02456572651863098, 0.10678958892822266, 0.09198499470949173, 0.07593625783920288, 0.14305391907691956, 0.08538073301315308, -0.04111288860440254, -0.01790003478527069, -0.0024679217021912336, 0.03383166715502739, -0.042955491691827774, -0.031683653593063354, 0.02778710424900055, -0.053109776228666306, -0.01570277474820614, 0.05444611981511116, -0.10807257145643234, -0.00015841526328586042, -0.014925065450370312, -0.005304206162691116, -0.08184773474931717, 0.028276974335312843, 0.04506155475974083, 0.0336410216987133, 0.03745109215378761, 0.011506076902151108, -0.0010400579776614904, 0.030190249904990196, -0.03108755685389042, -0.05174277722835541, 0.04629286006093025, 0.02810818701982498, 0.057049717754125595, -0.008443426340818405, -0.002984145190566778, -0.004514682572335005, -0.08842809498310089, 0.015178537927567959, -0.011800403706729412, -0.10274932533502579, -0.028994854539632797, -0.005422495771199465, 0.0402238555252552, -0.007638462353497744, -0.004548679571598768, 0.043599262833595276, 0.06585025787353516, -0.07177790999412537, 0.038183342665433884, 0.000316926947562024, -0.023010196164250374, -0.018119389191269875, 0.12755806744098663, -0.029702570289373398, -0.01015652995556593, -0.059056248515844345, 0.0947175845503807, 0.02847215160727501, -0.04676135629415512, -0.0011484554270282388, 0.07145674526691437, 0.06743879616260529, -0.10943575948476791, -0.04018706828355789, 0.06428363174200058, 0.06146806478500366, -0.025103546679019928, -0.08624499291181564, 0.004846961237490177, -0.05238010734319687, -0.057486120611429214, -0.056318849325180054, -0.03491798788309097, -0.09572737663984299, 0.11231037229299545, 0.07101139426231384, -0.10874102264642715, -0.07361601293087006, 0.08110713213682175, -0.0064906710758805275, 0.05368616059422493, -0.0018349586753174663, -0.0250141192227602, -0.022014975547790527, 0.05952775478363037, 0.022679883986711502, 0.03440539166331291, 0.05801156908273697, -0.016261614859104156, -1.9267138995360256e-08, -0.020924469456076622, 0.00782059133052826, 0.007650299929082394, -0.08766217529773712, -0.03859851136803627, 0.061062730848789215, -0.02243957482278347, -0.005004537757486105, 0.010417965240776539, -0.033607367426157, 0.0068754893727600574, 0.04897266626358032, -0.05267562344670296, 5.672816405422054e-05, 0.08323980867862701, 0.0012997715966776013, -0.06343550235033035, -0.05276073142886162, -0.0064402795396745205, -0.051372043788433075, -0.06017841026186943, 0.020528385415673256, -0.06906644999980927, -0.04046109691262245, 0.013538027182221413, -0.06068901717662811, -0.003798676887527108, -0.07600800693035126, 0.009271702729165554, -0.007799104787409306, 0.029246626421809196, 0.004852213431149721, 0.09407801926136017, 0.056014347821474075, 0.019805116578936577, -0.04717255383729935, 0.0494965985417366, 0.01954321376979351, -0.11856653541326523, -0.052785418927669525, -0.009882924146950245, -0.017975447699427605, -0.041780877858400345, -0.05374239757657051, -0.11366232484579086, -0.09755975753068924, 0.0486016608774662, 0.003404095536097884, -0.07665853202342987, 0.03216850012540817, -0.013870303519070148, -0.05115455016493797, -0.030844826251268387, -0.04256793484091759, -0.05529716610908508, 0.06646580994129181, -0.062231309711933136, 0.029709964990615845, 0.02504698745906353, -0.038647208362817764, -0.005119360517710447, 0.0008893350604921579, 0.06500400602817535, 0.06179634854197502]"
}
},
{
"name": "db.chroma.query.segment._query.query_embeddings.1",
"timestamp": "2024-01-23T21:47:09.046501Z",
"attributes": {
"embeddings": "[-0.021167762577533722, -0.03439260646700859, 0.0058017512783408165, -0.007966647855937481, 0.02376502752304077, -0.031745173037052155, -0.012614408507943153, -0.06414493173360825, 0.020165633410215378, -0.08882297575473785, -0.01786126010119915, -0.0681491494178772, -0.014057029038667679, -0.04547575116157532, 0.052258942276239395, 0.08119107037782669, -0.01767161674797535, -0.04887744039297104, 0.012606324627995491, -0.013008194044232368, -0.006311077158898115, 0.03886744752526283, 0.05800182744860649, 0.10872285068035126, 0.05640159547328949, -0.0988892912864685, -0.06236724182963371, 0.08906788378953934, -0.003973393701016903, 0.013015585020184517, 0.013766579329967499, -0.08719684928655624, -0.018849939107894897, 0.052878160029649734, -0.04795434698462486, 0.018592363223433495, 0.003259101649746299, 0.06537959724664688, -0.06702828407287598, 0.041294485330581665, 0.07399819046258926, -0.05151427164673805, 0.04187088459730148, 0.008416556753218174, -0.03619104623794556, 0.013703054748475552, -0.0034689228050410748, 0.05873261019587517, 0.0006399548146873713, 0.1366840898990631, 0.03454408422112465, -0.051180314272642136, 0.0252225361764431, 0.009749420918524265, 0.1408623456954956, -0.04582150653004646, -0.05453966557979584, 0.059339575469493866, -0.018907101824879646, 0.04133671894669533, 0.008158337324857712, -0.06166509911417961, 0.02156376838684082, 0.05868002399802208, 0.038592997938394547, -0.029848432168364525, 0.05385807901620865, -0.07630190998315811, -0.050351351499557495, 0.0011880360543727875, -0.03309452533721924, -0.05798250809311867, 0.03359082713723183, 0.05653802677989006, 0.04601285234093666, 0.05540644749999046, 0.01185417827218771, -0.06335807591676712, 0.0012864789459854364, 0.08841302990913391, -0.016453653573989868, 0.0427200011909008, 0.0316358245909214, 0.047262273728847504, -0.05914708226919174, -0.03422379121184349, 0.04035981744527817, -0.025106485933065414, -0.05876951664686203, -0.027694309130311012, 0.019040485844016075, -0.03460255637764931, -0.05918477103114128, 0.016780341044068336, -0.046335868537425995, 0.05575210228562355, 0.004100665915757418, 0.012866304256021976, -0.05400334671139717, -0.03429311513900757, -0.06431403756141663, 0.04240953549742699, -0.03721174597740173, -0.0810861736536026, -0.04848036915063858, -0.05714283883571625, 0.026641622185707092, 0.01758853904902935, -0.11260650306940079, 0.014960172586143017, 0.019847314804792404, -0.0723264291882515, 0.02661767788231373, 0.001284053549170494, -0.007888894528150558, 0.04648272693157196, -0.08495215326547623, -0.07271622866392136, -0.0791686624288559, -0.05127555504441261, -0.09453310072422028, -0.05035112798213959, 0.09153077751398087, 0.07441414892673492, -0.01406773366034031, -0.012814546935260296, 0.008186708204448223, -2.550197341555528e-33, 0.04130001366138458, -0.060710594058036804, -0.015049715526401997, 0.04045252874493599, 0.0557674765586853, -0.08132638037204742, -0.05763687565922737, -0.0060446010902523994, -0.054270148277282715, -0.021341724321246147, 0.0681234821677208, -0.04240187257528305, 0.052468929439783096, -0.07120761275291443, 0.004225547891110182, -0.04063575714826584, -0.012352929450571537, -0.023929676041007042, -0.018602101132273674, -0.004154928959906101, -0.023531541228294373, -0.034135304391384125, -0.04750525578856468, 0.010172168724238873, -0.031765613704919815, 0.08414985239505768, 0.021447015926241875, 0.025972656905651093, 0.0012261528754606843, 0.006656584795564413, -0.10672400146722794, -0.017840875312685966, -0.017880430445075035, -0.08224820345640182, -0.02716796286404133, -0.008427073247730732, -0.028094017878174782, -0.019784502685070038, 0.0700279250741005, -0.029444316402077675, -0.049080025404691696, 0.023307764902710915, 0.037553563714027405, -0.08113836497068405, 0.021111363545060158, 0.017380930483341217, -0.03756500408053398, 0.044616300612688065, -0.02021588571369648, -0.035405635833740234, 0.06681307405233383, -0.012360861524939537, 0.029090238735079765, 0.026111489161849022, -0.05895277485251427, -0.007940702140331268, -0.0841827467083931, -0.008791148662567139, -0.07336302846670151, 0.013196446932852268, -0.02187541127204895, -0.07236422598361969, 0.005393237341195345, 0.00682192062959075, -0.031029723584651947, -0.030586279928684235, 0.032504476606845856, -0.07072892785072327, -0.01813017763197422, 0.031862854957580566, 0.03811290115118027, -0.03587355837225914, 0.0959530621767044, -0.026945143938064575, 0.0004465418460313231, 0.07757928967475891, 0.07206105440855026, 0.044085923582315445, 0.03536035493016243, 0.10247663408517838, -0.06628499180078506, -0.008462646044790745, -0.06628113985061646, 0.004388345871120691, 0.018969444558024406, -0.0717795267701149, 0.017673902213573456, -0.053256548941135406, 0.016331428661942482, -0.005883345380425453, 0.04394875466823578, 0.05145733058452606, 0.03457028418779373, -0.03480254113674164, 0.05082661285996437, -1.0038526146415519e-33, -0.009893221780657768, -0.03008336015045643, 0.03563648834824562, -0.15457294881343842, 0.012237786315381527, 0.020480534061789513, 0.04366731643676758, -0.044884566217660904, 0.06599174439907074, 0.003979774657636881, 0.11247958242893219, 0.010472172871232033, -0.056293390691280365, -0.04810985177755356, -0.02875439263880253, -0.05802953988313675, 0.005881960969418287, -0.030452407896518707, 0.006983831990510225, 0.016244150698184967, 0.11564341187477112, 0.09782040864229202, -0.02106589265167713, -0.0027237364556640387, -0.048340797424316406, 0.023100953549146652, -0.01901528425514698, 0.09837326407432556, 0.05735604092478752, 0.04899923503398895, -0.013888477347791195, 0.08989644050598145, -0.01158017199486494, 0.06197698041796684, -0.04763267934322357, 0.0019929069094359875, -0.04740643873810768, -0.033345844596624374, -0.01131925918161869, 0.0034372394438833, 0.04213418439030647, 0.03559784218668938, -0.062017131596803665, -0.05756337568163872, 0.06403703987598419, 0.05556986480951309, 0.03860724717378616, 0.06643757224082947, 0.04062255844473839, -0.07947801053524017, -0.046062298119068146, 0.0890393927693367, -0.05893469229340553, 0.019603334367275238, 0.023877590894699097, 0.0671909898519516, 0.003432287834584713, -0.006425967440009117, -0.0121149942278862, -0.005697814282029867, -0.08577165007591248, 0.06238243728876114, -0.006197126116603613, 0.025844678282737732, -0.07390890270471573, 0.04225705564022064, 0.03610927239060402, -0.0838417261838913, 0.03854922577738762, 0.015916520729660988, 0.09681838005781174, 0.046653568744659424, -0.05995472893118858, 0.013915362767875195, -0.02628212794661522, -0.02587665617465973, -0.006469472777098417, 0.026824476197361946, -0.0824342593550682, 0.03616642206907272, -0.05064127594232559, 0.08353569358587265, -0.020785760134458542, -0.05099663510918617, 0.09417621046304703, 0.027563124895095825, 0.011122556403279305, 0.04190441593527794, -0.0870526060461998, 0.024611299857497215, -0.09531863033771515, -0.015931613743305206, -0.02929593250155449, 0.03589509800076485, -0.13687725365161896, -1.7230991744554558e-08, -0.04787730053067207, -0.027671203017234802, 0.0924193263053894, -0.10821261256933212, -0.0293410774320364, 0.10089648514986038, -0.05155133455991745, 0.05188142880797386, -0.011570318602025509, -0.031686633825302124, 0.04266354814171791, 0.08536260575056076, -0.050784528255462646, -0.00491418968886137, 0.03505030274391174, 0.01876073144376278, -0.011717832647264004, -0.01548430323600769, -0.019486261531710625, -0.0083812540397048, -0.032196104526519775, -0.042530205100774765, 0.0008490110049024224, -0.03290144354104996, -0.08145955950021744, 0.08804035931825638, -0.022640109062194824, 0.0617789700627327, -0.02675469219684601, -0.015017855912446976, 0.10546790808439255, 0.00010206511797150597, 0.09494058042764664, -0.0057518682442605495, 0.03723328560590744, 0.01388932578265667, 0.016926202923059464, 0.015854861587285995, 0.008203963749110699, 0.10948973894119263, -0.004191818181425333, -0.034896571189165115, 0.06970661878585815, 0.011053844355046749, -0.0457465685904026, 0.032908327877521515, 0.0348767451941967, -0.05684136599302292, 0.0672527477145195, 0.0216323621571064, 0.032846588641405106, -0.044264260679483414, -0.003992707468569279, -0.031409405171871185, -0.038772791624069214, -0.011668460443615913, -0.03870437294244766, -0.027680059894919395, 0.08444681018590927, 0.052766796201467514, 0.10742847621440887, -0.03799748420715332, 0.1138821393251419, 0.014279183931648731]"
}
}
],
"links": [],
"resource": {
"attributes": {},
"schema_url": ""
}
}
{
"name": "chroma.query",
"context": {
"trace_id": "0xf348efdbe785092acf97a0253dded059",
"span_id": "0xed5d9474bafbc7d3",
"trace_state": "[]"
},
"kind": "SpanKind.INTERNAL",
"parent_id": "0x6c70b3b35f07da2f",
"start_time": "2024-01-23T21:47:09.039801Z",
"end_time": "2024-01-23T21:47:09.047895Z",
"status": {
"status_code": "UNSET"
},
"attributes": {
"traceloop.workflow.name": "assess_claims",
"db.system": "chroma",
"db.operation": "query",
"db.chroma.query.query_texts_count": 2,
"db.chroma.query.n_results": 3,
"db.chroma.query.include": "['documents', 'distances']"
},
"events": [
{
"name": "db.chroma.query.result.0",
"timestamp": "2024-01-23T21:47:09.047876Z",
"attributes": {
"ids": [
"36474",
"4983",
"19238"
],
"distances": [
1.7072678804397583,
1.7307902574539185,
1.7318366765975952
],
"metadata": [],
"documents": [
"Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) requires efficient methods for genetic modification. However, techniques to generate cell type\u2013specific lineage reporters, as well as reliable tools to disrupt, repair or overexpress genes by gene targeting, are inefficient at best and thus are not routinely used. Here we report the highly efficient targeting of three genes in human pluripotent cells using zinc-finger nuclease (ZFN)\u2013mediated genome editing. First, using ZFNs specific for the OCT4 (POU5F1) locus, we generated OCT4-eGFP reporter cells to monitor the pluripotent state of hESCs. Second, we inserted a transgene into the AAVS1 locus to generate a robust drug-inducible overexpression system in hESCs. Finally, we targeted the PITX3 gene, demonstrating that ZFNs can be used to generate reporter cells by targeting non-expressed genes in hESCs and hiPSCs.",
"Microstructural development of human newborn cerebral white matter assessed in vivo by diffusion tensor magnetic resonance imaging.. Alterations of the architecture of cerebral white matter in the developing human brain can affect cortical development and result in functional disabilities. A line scan diffusion-weighted magnetic resonance imaging (MRI) sequence with diffusion tensor analysis was applied to measure the apparent diffusion coefficient, to calculate relative anisotropy, and to delineate three-dimensional fiber architecture in cerebral white matter in preterm (n = 17) and full-term infants (n = 7). To assess effects of prematurity on cerebral white matter development, early gestation preterm infants (n = 10) were studied a second time at term. In the central white matter the mean apparent diffusion coefficient at 28 wk was high, 1.8 microm2/ms, and decreased toward term to 1.2 microm2/ms. In the posterior limb of the internal capsule, the mean apparent diffusion coefficients at both times were similar (1.2 versus 1.1 microm2/ms). Relative anisotropy was higher the closer birth was to term with greater absolute values in the internal capsule than in the central white matter. Preterm infants at term showed higher mean diffusion coefficients in the central white matter (1.4 +/- 0.24 versus 1.15 +/- 0.09 microm2/ms, p = 0.016) and lower relative anisotropy in both areas compared with full-term infants (white matter, 10.9 +/- 0.6 versus 22.9 +/- 3.0%, p = 0.001; internal capsule, 24.0 +/- 4.44 versus 33.1 +/- 0.6% p = 0.006). Nonmyelinated fibers in the corpus callosum were visible by diffusion tensor MRI as early as 28 wk; full-term and preterm infants at term showed marked differences in white matter fiber organization. The data indicate that quantitative assessment of water diffusion by diffusion tensor MRI provides insight into microstructural development in cerebral white matter in living infants.",
"The human myelin basic protein gene is included within a 179-kilobase transcription unit: expression in the immune and central nervous systems.. Two human Golli (for gene expressed in the oligodendrocyte lineage)-MBP (for myelin basic protein) cDNAs have been isolated from a human oligodendroglioma cell line. Analysis of these cDNAs has enabled us to determine the entire structure of the human Golli-MBP gene. The Golli-MBP gene, which encompasses the MBP transcription unit, is approximately 179 kb in length and consists of 10 exons, seven of which constitute the MBP gene. The human Golli-MBP gene contains two transcription start sites, each of which gives rise to a family of alternatively spliced transcripts. At least two Golli-MBP transcripts, containing the first three exons of the gene and one or more MBP exons, are produced from the first transcription start site. The second family of transcripts contains only MBP exons and produces the well-known MBPs. In humans, RNA blot analysis revealed that Golli-MBP transcripts were expressed in fetal thymus, spleen, and human B-cell and macrophage cell lines, as well as in fetal spinal cord. These findings clearly link the expression of exons encoding the autoimmunogen/encephalitogen MBP in the central nervous system to cells and tissues of the immune system through normal expression of the Golli-MBP gene. They also establish that this genetic locus, which includes the MBP gene, is conserved among species, providing further evidence that the MBP transcription unit is an integral part of the Golli transcription unit and suggest that this structural arrangement is important for the genetic function and/or regulation of these genes."
]
}
},
{
"name": "db.chroma.query.result.1",
"timestamp": "2024-01-23T21:47:09.047891Z",
"attributes": {
"ids": [
"5836",
"36474",
"33370"
],
"distances": [
1.4618057012557983,
1.5080177783966064,
1.5208396911621094
],
"metadata": [],
"documents": [
"Induction of myelodysplasia by myeloid-derived suppressor cells.. Myelodysplastic syndromes (MDS) are age-dependent stem cell malignancies that share biological features of activated adaptive immune response and ineffective hematopoiesis. Here we report that myeloid-derived suppressor cells (MDSC), which are classically linked to immunosuppression, inflammation, and cancer, were markedly expanded in the bone marrow of MDS patients and played a pathogenetic role in the development of ineffective hematopoiesis. These clonally distinct MDSC overproduce hematopoietic suppressive cytokines and function as potent apoptotic effectors targeting autologous hematopoietic progenitors. Using multiple transfected cell models, we found that MDSC expansion is driven by the interaction of the proinflammatory molecule S100A9 with CD33. These 2 proteins formed a functional ligand/receptor pair that recruited components to CD33\u2019s immunoreceptor tyrosine-based inhibition motif (ITIM), inducing secretion of the suppressive cytokines IL-10 and TGF-\u03b2 by immature myeloid cells. S100A9 transgenic mice displayed bone marrow accumulation of MDSC accompanied by development of progressive multilineage cytopenias and cytological dysplasia. Importantly, early forced maturation of MDSC by either all-trans-retinoic acid treatment or active immunoreceptor tyrosine-based activation motif\u2013bearing (ITAM-bearing) adapter protein (DAP12) interruption of CD33 signaling rescued the hematologic phenotype. These findings indicate that primary bone marrow expansion of MDSC driven by the S100A9/CD33 pathway perturbs hematopoiesis and contributes to the development of MDS.",
"Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) requires efficient methods for genetic modification. However, techniques to generate cell type\u2013specific lineage reporters, as well as reliable tools to disrupt, repair or overexpress genes by gene targeting, are inefficient at best and thus are not routinely used. Here we report the highly efficient targeting of three genes in human pluripotent cells using zinc-finger nuclease (ZFN)\u2013mediated genome editing. First, using ZFNs specific for the OCT4 (POU5F1) locus, we generated OCT4-eGFP reporter cells to monitor the pluripotent state of hESCs. Second, we inserted a transgene into the AAVS1 locus to generate a robust drug-inducible overexpression system in hESCs. Finally, we targeted the PITX3 gene, demonstrating that ZFNs can be used to generate reporter cells by targeting non-expressed genes in hESCs and hiPSCs.",
"Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth. Glioblastomas are deadly cancers that display a functional cellular hierarchy maintained by self-renewing glioblastoma stem cells (GSCs). GSCs are regulated by molecular pathways distinct from the bulk tumor that may be useful therapeutic targets. We determined that A20 (TNFAIP3), a regulator of cell survival and the NF-kappaB pathway, is overexpressed in GSCs relative to non-stem glioblastoma cells at both the mRNA and protein levels. To determine the functional significance of A20 in GSCs, we targeted A20 expression with lentiviral-mediated delivery of short hairpin RNA (shRNA). Inhibiting A20 expression decreased GSC growth and survival through mechanisms associated with decreased cell-cycle progression and decreased phosphorylation of p65/RelA. Elevated levels of A20 in GSCs contributed to apoptotic resistance: GSCs were less susceptible to TNFalpha-induced cell death than matched non-stem glioma cells, but A20 knockdown sensitized GSCs to TNFalpha-mediated apoptosis. The decreased survival of GSCs upon A20 knockdown contributed to the reduced ability of these cells to self-renew in primary and secondary neurosphere formation assays. The tumorigenic potential of GSCs was decreased with A20 targeting, resulting in increased survival of mice bearing human glioma xenografts. In silico analysis of a glioma patient genomic database indicates that A20 overexpression and amplification is inversely correlated with survival. Together these data indicate that A20 contributes to glioma maintenance through effects on the glioma stem cell subpopulation. Although inactivating mutations in A20 in lymphoma suggest A20 can act as a tumor suppressor, similar point mutations have not been identified through glioma genomic sequencing: in fact, our data suggest A20 may function as a tumor enhancer in glioma through promotion of GSC survival. A20 anticancer therapies should therefore be viewed with caution as effects will likely differ depending on the tumor type."
]
}
}
],
"links": [],
"resource": {
"attributes": {},
"schema_url": ""
}
}
{
"name": "assess_claims.workflow",
"context": {
"trace_id": "0xf348efdbe785092acf97a0253dded059",
"span_id": "0x6c70b3b35f07da2f",
"trace_state": "[]"
},
"kind": "SpanKind.INTERNAL",
"parent_id": null,
"start_time": "2024-01-23T21:47:09.039750Z",
"end_time": "2024-01-23T21:47:09.048102Z",
"status": {
"status_code": "UNSET"
},
"attributes": {
"traceloop.workflow.name": "assess_claims",
"traceloop.span.kind": "workflow",
"traceloop.entity.name": "assess_claims"
},
"events": [],
"links": [],
"resource": {
"attributes": {},
"schema_url": ""
}
}
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment