A Photoshop CS5 script for making sprites out of a pile of layers :D Assumes that the top layer is the first frame, bottom is the last. Based on this one: http://www.garagegames.com/community/blogs/view/11527 I just like my sprites to run vertically. I'm weird like that.

Layers-to-Spritesheet Photoshop Script

if (documents.length > 0) 
{
  var docRef = activeDocument;    
	
	var activeLayer = docRef.activeLayer;

	numLayers = docRef.artLayers.length; 	
	
	var rows = numLayers;
	
 	var spriteX = docRef.width;
 	var spriteY = docRef.height;	
	
	app.preferences.rulerUnits = Units.PIXELS;
 	docRef.resizeCanvas( spriteX, spriteY * rows, AnchorPosition.BOTTOMLEFT );
 	 	
 	
 	for (i=0;i < numLayers ;i++) 
 	{ 	
		
 		docRef.artLayers[i].visible = true;
 		
  		var movY = spriteY*i;
  		
 		docRef.artLayers[i].translate(0, -movY);
 		
 
 	}
 
}else
{
msg("Sorry must have more than 1 image open in photoshop to work!");
}