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@rmostowy
Created June 21, 2024 10:17
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createdb file.fasta mmseqs/DB
MMseqs Version: 15-6f452
Database type 0
Shuffle input database true
Createdb mode 0
Write lookup file 1
Offset of numeric ids 0
Compressed 0
Verbosity 3
Converting sequences
[405] 0s 31ms
Time for merging to DB_h: 0h 0m 0s 3ms
Time for merging to DB: 0h 0m 0s 11ms
Database type: Nucleotide
Time for processing: 0h 0m 0s 74ms
Create directory mmseqs/tmp
search mmseqs/DB mmseqs/DB mmseqs/resultDB mmseqs/tmp --search-type 3 --threads 8 --sub-mat blastn-scoring.out --seed-sub-mat blastn-scoring.out -s 7.5 --prefilter-mode 2 -e 1e-3
MMseqs Version: 15-6f452
Substitution matrix aa:blastn-scoring.out,nucl:blastn-scoring.out
Add backtrace false
Alignment mode 3
Alignment mode 0
Allow wrapped scoring false
E-value threshold 0.001
Seq. id. threshold 0
Min alignment length 0
Seq. id. mode 0
Alternative alignments 0
Coverage threshold 0
Coverage mode 0
Max sequence length 10000
Compositional bias 1
Compositional bias 1
Max reject 2147483647
Max accept 2147483647
Include identical seq. id. false
Preload mode 0
Pseudo count a substitution:1.100,context:1.400
Pseudo count b substitution:4.100,context:5.800
Score bias 0
Realign hits false
Realign score bias -0.2
Realign max seqs 2147483647
Correlation score weight 0
Gap open cost aa:11,nucl:5
Gap extension cost aa:1,nucl:2
Zdrop 40
Threads 8
Compressed 0
Verbosity 3
Seed substitution matrix aa:blastn-scoring.out,nucl:blastn-scoring.out
Sensitivity 7.5
k-mer length 15
Target search mode 0
k-score seq:2147483647,prof:2147483647
Alphabet size aa:21,nucl:5
Max results per query 300
Split database 0
Split mode 2
Split memory limit 0
Diagonal scoring true
Exact k-mer matching 1
Mask residues 1
Mask residues probability 0.9
Mask lower case residues 0
Minimum diagonal score 15
Selected taxa
Spaced k-mers 1
Spaced k-mer pattern
Local temporary path
Rescore mode 0
Remove hits by seq. id. and coverage false
Sort results 0
Mask profile 1
Profile E-value threshold 0.1
Global sequence weighting false
Allow deletions false
Filter MSA 1
Use filter only at N seqs 0
Maximum seq. id. threshold 0.9
Minimum seq. id. 0.0
Minimum score per column -20
Minimum coverage 0
Select N most diverse seqs 1000
Pseudo count mode 0
Min codons in orf 30
Max codons in length 32734
Max orf gaps 2147483647
Contig start mode 2
Contig end mode 2
Orf start mode 1
Forward frames 1,2,3
Reverse frames 1,2,3
Translation table 1
Translate orf 0
Use all table starts false
Offset of numeric ids 0
Create lookup 0
Add orf stop false
Overlap between sequences 0
Sequence split mode 1
Header split mode 0
Chain overlapping alignments 0
Merge query 1
Search type 3
Search iterations 1
Start sensitivity 4
Search steps 1
Prefilter mode 2
Exhaustive search mode false
Filter results during exhaustive search 0
Strand selection 2
LCA search mode false
Disk space limit 0
MPI runner
Force restart with latest tmp false
Remove temporary files false
splitsequence mmseqs/DB mmseqs/tmp/6157013917641327162/target_seqs_split --max-seq-len 10000 --sequence-overlap 0 --sequence-split-mode 1 --headers-split-mode 0 --create-lookup 0 --threads 8 --compressed 0 -v 3
[=================================================================] 100.00% 500 0s 2ms
Time for merging to target_seqs_split_h: 0h 0m 0s 3ms
Time for merging to target_seqs_split: 0h 0m 0s 2ms
Time for processing: 0h 0m 0s 19ms
extractframes mmseqs/DB mmseqs/tmp/6157013917641327162/query_seqs --forward-frames 1 --reverse-frames 1 --create-lookup 0 --threads 8 --compressed 0 -v 3
[=================================================================] 100.00% 500 0s 15ms
Time for merging to query_seqs_h: 0h 0m 0s 1ms
Time for merging to query_seqs: 0h 0m 0s 25ms
Time for processing: 0h 0m 0s 69ms
splitsequence mmseqs/tmp/6157013917641327162/query_seqs mmseqs/tmp/6157013917641327162/query_seqs_split --max-seq-len 10000 --sequence-overlap 0 --sequence-split-mode 1 --headers-split-mode 0 --create-lookup 0 --threads 8 --compressed 0 -v 3
[=================================================================] 100.00% 1.00K 0s 1ms
Time for merging to query_seqs_split_h: 0h 0m 0s 2ms
Time for merging to query_seqs_split: 0h 0m 0s 2ms
Time for processing: 0h 0m 0s 11ms
align mmseqs/tmp/6157013917641327162/query_seqs_split mmseqs/tmp/6157013917641327162/target_seqs_split mmseqs/tmp/6157013917641327162/search/pref_0 mmseqs/tmp/6157013917641327162/aln --sub-mat 'aa:blastn-scoring.out,nucl:blastn-scoring.out' -a 0 --alignment-mode 3 --alignment-output-mode 0 --wrapped-scoring 0 -e 0.001 --min-seq-id 0 --min-aln-len 0 --seq-id-mode 0 --alt-ali 0 -c 0 --cov-mode 0 --max-seq-len 10000 --comp-bias-corr 1 --comp-bias-corr-scale 1 --max-rejected 2147483647 --max-accept 2147483647 --add-self-matches 0 --db-load-mode 0 --pca substitution:1.100,context:1.400 --pcb substitution:4.100,context:5.800 --score-bias 0 --realign 0 --realign-score-bias -0.2 --realign-max-seqs 2147483647 --corr-score-weight 0 --gap-open aa:11,nucl:5 --gap-extend aa:1,nucl:2 --zdrop 40 --threads 8 --compressed 0 -v 3
Compute score, coverage and sequence identity
Query database size: 7214 type: Nucleotide
Target database size: 3607 type: Nucleotide
Calculation of alignments
[=================================================================] 100.00% 7.21K 4m 4s 688ms
Time for merging to aln: 0h 0m 0s 2ms
26020898 alignments calculated
5185 sequence pairs passed the thresholds (0.000199 of overall calculated)
0.718741 hits per query sequence
Time for processing: 0h 4m 4s 909ms
offsetalignment mmseqs/DB mmseqs/tmp/6157013917641327162/query_seqs_split mmseqs/DB mmseqs/tmp/6157013917641327162/target_seqs_split mmseqs/tmp/6157013917641327162/aln mmseqs/resultDB --chain-alignments 0 --merge-query 1 --search-type 3 --threads 8 --compressed 0 --db-load-mode 0 -v 3
Computing ORF lookup
Computing contig offsets
Computing contig lookup
Time for contig lookup: 0h 0m 0s 0ms
Writing results to: mmseqs/resultDB
[=================================================================] 100.00% 500 0s 2ms
Time for merging to resultDB: 0h 0m 0s 0ms
Time for processing: 0h 0m 0s 7ms
convertalis mmseqs/DB mmseqs/DB mmseqs/resultDB mmseqs/result.m8
MMseqs Version: 15-6f452
Substitution matrix aa:blosum62.out,nucl:nucleotide.out
Alignment format 0
Format alignment output query,target,fident,alnlen,mismatch,gapopen,qstart,qend,tstart,tend,evalue,bits
Translation table 1
Gap open cost aa:11,nucl:5
Gap extension cost aa:1,nucl:2
Database output false
Preload mode 0
Search type 0
Threads 12
Compressed 0
Verbosity 3
[=================================================================] 100.00% 500 0s 3ms
Time for merging to result.m8: 0h 0m 0s 3ms
Time for processing: 0h 0m 0s 11ms
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