For the review of the article (https://f1000research.com/articles/9-1380). A macro to test the option "disable smoothing" of the plugin "Process>Subtract Background..."

test_optionDisableSmoothing.ijm

run("Close All");

run("Blobs (25K)");
img1 = getImageID();
run("Invert LUT");
run("32-bit");

rename("On_disableSmoothing");
run("Duplicate...", "title=Off_disableSmoothing");
img2 = getImageID();

selectImage(img1);
run("Subtract Background...", "rolling=50 disable");
run("Histogram", "bins=256 use x_min=-20 x_max=300 y_max=Auto");

selectImage(img2);
run("Subtract Background...", "rolling=50 ");
run("Histogram", "bins=256 use x_min=-20 x_max=300 y_max=Auto");

run("Tile");