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Shorthand	Strain	Notes	Paper	Table
Gallagher ... Manoil	5075	Most popular transposon library in Abau. References: Wang ... Mandel, Hauser	Gallagher et al (2015)	Presence means candidacy
Geisinger ... Opijnen, Isberg	17978	Best coverage; Tn chemical genomics	Geisinger et al (2020)	Column F
Wang ... Mandel, Hauser	17978	Poor coverage/sequencing/alignment; Tn-seq	Wang et al (2014)	[Tab A](https://journals-asm-org.ezproxy.library.wisc.edu/doi/suppl/10.

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Standardizing the Methodology to Bottleneck Analysis

This method is an attempt to formalize the intial development from this GitHub repository, which is based on the methodologies from this Nature Methods paper.

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The GitHub code is attempting to estimate bottleneck sizes in bacterial populations, a concept derived from population genetics. It makes use of R and statistical equations to estimate $( N_b )$, the bottleneck size, based on temporal allele frequency data. The Nature Methods paper it draws inspiration from elaborates on this technique.

T0 Explanation

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Protocol for Library Growth with LB Media

Introduction

This protocol outlines the steps to grow up your libraries and perform chemical genomics for sequence quantification.

Materials

• Library
• Culture tubes
• Eppitubes

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Protocol for Antibiotic Checkerboard Assay

I. Preparation of Antibiotic Serial Dilutions

1. Add 200µL of DRUG A (100x) and 300µL of LB into the top-right well of a deep well plate, yielding a 40x concentration in a total volume of 500µL.
2. Fill the rest of the wells in the top row with 250µL of LB.
3. Perform a serial dilution across the top row by transferring 250µL sequentially from one well to the next.
4. Discard 250µL from the penultimate well.
5. Repeat steps 1-4 in the bottom-left well using DRUG B instead of DRUG A.

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#!/usr/bin/env bash

function get_headphones_index() {
  echo $(pacmd list-cards | grep bluez_card -B1 | grep index | awk '{print $2}')
}

function get_headphones_mac_address() {
  local temp=$(pacmd list-cards | grep bluez_card -C20 | grep 'device.string' | cut -d' ' -f 3)
  temp="${temp%\"}"
  temp="${temp#\"}"

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= Arch Linux step-by-step installation =
= http://blog.fabio.mancinelli.me/2012/12/28/Arch_Linux_on_BTRFS.html =


== Boot the installation CD ==

== Create partition ==

cfdisk /dev/sda
 * Create a partition with code 8300 (Linux)