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Working on Acinetobacter

Ryan Ward ryandward

Working on Acinetobacter
  • Las Cruces & Madison
  • 19:22 (UTC -05:00)
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Standardizing the Methodology to Bottleneck Analysis

This method is an attempt to formalize the intial development from this GitHub repository, which is based on the methodologies from this Nature Methods paper.

The GitHub code is attempting to estimate bottleneck sizes in bacterial populations, a concept derived from population genetics. It makes use of R and statistical equations to estimate $( N_b )$, the bottleneck size, based on temporal allele frequency data. The Nature Methods paper it draws inspiration from elaborates on this technique.

T0 Explanation

ryandward / Library
Last active September 22, 2023 17:28
Library Growth
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View Protocol for Checkerboard

Protocol for Antibiotic Checkerboard Assay

I. Preparation of Antibiotic Serial Dilutions

  1. Add 200µL of DRUG A (100x) and 300µL of LB into the top-right well of a deep well plate, yielding a 40x concentration in a total volume of 500µL.
  2. Fill the rest of the wells in the top row with 250µL of LB.
  3. Perform a serial dilution across the top row by transferring 250µL sequentially from one well to the next.
  4. Discard 250µL from the penultimate well.
  5. Repeat steps 1-4 in the bottom-left well using DRUG B instead of DRUG A.
ryandward /
Last active September 26, 2020 03:50 — forked from egelev/
Connect bluetooth headphones on Arch Linux (Gnome settings can't do it)
#!/usr/bin/env bash
function get_headphones_index() {
echo $(pacmd list-cards | grep bluez_card -B1 | grep index | awk '{print $2}')
function get_headphones_mac_address() {
local temp=$(pacmd list-cards | grep bluez_card -C20 | grep 'device.string' | cut -d' ' -f 3)
View Arch Linux step-by-step installation on btrfs
= Arch Linux step-by-step installation =
= =
== Boot the installation CD ==
== Create partition ==
cfdisk /dev/sda
* Create a partition with code 8300 (Linux)