This protocol outlines the steps to grow up your libraries and perform chemical genomics for sequence quantification.
- Library
- Culture tubes
- Eppitubes
The calculation of bottleneck size (
This method is an attempt to formalize the intial development from this GitHub repository, which is based on the methodologies from this Nature Methods paper.
Shorthand | Strain | Notes | Paper | Table |
---|---|---|---|---|
Gallagher ... Manoil | 5075 | Most popular transposon library in Abau. References: Wang ... Mandel, Hauser | Gallagher et al (2015) | Presence means candidacy |
Geisinger ... Opijnen, Isberg | 17978 | Best coverage; Tn chemical genomics | Geisinger et al (2020) | Column F |
Wang ... Mandel, Hauser | 17978 | Poor coverage/sequencing/alignment; Tn-seq | Wang et al (2014) | [Tab A](https://journals-asm-org.ezproxy.library.wisc.edu/doi/suppl/10. |
#!/usr/bin/env bash | |
function get_headphones_index() { | |
echo $(pacmd list-cards | grep bluez_card -B1 | grep index | awk '{print $2}') | |
} | |
function get_headphones_mac_address() { | |
local temp=$(pacmd list-cards | grep bluez_card -C20 | grep 'device.string' | cut -d' ' -f 3) | |
temp="${temp%\"}" | |
temp="${temp#\"}" |
= Arch Linux step-by-step installation = | |
= http://blog.fabio.mancinelli.me/2012/12/28/Arch_Linux_on_BTRFS.html = | |
== Boot the installation CD == | |
== Create partition == | |
cfdisk /dev/sda | |
* Create a partition with code 8300 (Linux) |