See https://forum.image.sc/t/error-when-trying-to-write-ome-tiff-using-python-bioformats/21543

generate_tiff.py

import bioformats.omexml as ome
import javabridge as jv
import bioformats
import os
import sys
import numpy as np

# Write file to disk
def writeOMETIFF(img_XYCZT, path, type='uint16', verbose=True):

    if verbose:
        print('Dimensions XYCZT: ' + str(np.shape(img_XYCZT)))
        sys.stdout.flush()

    # Get the new dimensions
    SizeX = np.shape(img_XYCZT)[0]
    SizeY = np.shape(img_XYCZT)[1]
    SizeC = np.shape(img_XYCZT)[2]
    SizeZ = np.shape(img_XYCZT)[3]
    SizeT = np.shape(img_XYCZT)[4]

    # Start JVM for bioformats
    bfpackage = r'/usr/local/Cellar/bioformats/5.9.0/libexec/bioformats_package.jar'
    jars = jv.JARS + [bfpackage]
    jv.start_vm(class_path=jars, run_headless=True, max_heap_size='4G')

    # Getting metadata info
    omexml = ome.OMEXML()
    omexml.image(0).Name = os.path.split(path)[1]
    p = omexml.image(0).Pixels
    assert isinstance(p, ome.OMEXML.Pixels)
    p.SizeX = SizeX
    p.SizeY = SizeY
    p.SizeC = SizeC
    p.SizeT = SizeT
    p.SizeZ = SizeZ
    p.DimensionOrder = ome.DO_XYCZT
    p.PixelType = type
    p.channel_count = SizeC
    p.plane_count = SizeZ
    p.Channel(0).SamplesPerPixel = SizeC
    omexml.structured_annotations.add_original_metadata(ome.OM_SAMPLES_PER_PIXEL, str(SizeC))

    # Converting to omexml
    xml = omexml.to_xml()

    # Write file using Bioformats
    for frame in range(SizeT):

        index = frame
        pixel_buffer = bioformats.formatwriter.convert_pixels_to_buffer(img_XYCZT[:, :, :, :, frame], type)
        script = """
        importClass(Packages.loci.formats.services.OMEXMLService,
                    Packages.loci.common.services.ServiceFactory,
                    Packages.loci.formats.out.TiffWriter);
    
        var service = new ServiceFactory().getInstance(OMEXMLService);
        var metadata = service.createOMEXMLMetadata(xml);
        var writer = new TiffWriter();
        writer.setBigTiff(true);
        writer.setMetadataRetrieve(metadata);
        writer.setId(path);
        writer.setInterleaved(true);
        writer.saveBytes(index, buffer);
        writer.close();
        """
        jv.run_script(script, dict(path=path, xml=xml, index=index, buffer=pixel_buffer))

##################################################################################################################

# Dimension TZCXY
SizeT = 30
SizeZ = 23
SizeC = 2
SizeX = 217
SizeY = 94

output_file = r'stackome.tiff'
img5d = np.random.randn(SizeX, SizeY, SizeC, SizeZ, SizeT).astype(np.uint16)
writeOMETIFF(img5d, output_file, type='uint16', verbose=True)