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scientificprotocols/protocol.md

Last active August 29, 2015 14:03
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EPON resin mixture for transmission electron microscopy
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protocol.md

For Epon WPE 153:

Table 1

Prepare mixture A and mixture B in 30 - 120 ml batches. Mix well and store in glass scintillation vials or some other container that can be capped tightly. Store at -20 degrees C. The mixtures can be stored for years.

For use:

  1. Be certain to completely warm the vials before opening them to prevent water from mixing with the plastic.
  • Mix equal volumes of A and B together.
  • Then add 0.1 ml of DMP-30 for each 5 ml of plastic.
  • Mix well and store no longer than a day at room temp. I usually put the remainder of the plastic in a tube or vial, cap and wrap the cap with parafilm, and put it at 4 degrees C overnight.

DOI

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