A method of aortic ring assay to analyze micro-vessel outgrowth for angiogenesis analysis.
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- Cover a 48-well plate with Matrigel (100μl/well) of and incubate for 30 min at 37°C, 5% CO2.
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- Sacrifice the 1-2 month old mice/rats (WT/mutant) and excise thoracic aorta. Transfer the aorta into a dish with sterilized 1x PBS buffer. After removing the fibroadipose tissue, section arteries into 1-1.5 mm long cross sections, rinse the sections five times with endothelial cell growth medium (ECGM), and place them on the Matrigel-coated wells.
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- Cover the artery rings with an additional 100μl of Matrigel. If there are some bubbles, use pipette to remove them. Incubate the rings in 1.5 ml of ECGM medium.