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An example SPAGeDi result file
SPAGeDi 1.4c (build 17-07-2013) - a program for Spatial Pattern Analysis of Genetic Diversity
Written by Olivier Hardy & Xavier Vekemans
Contributions by Reed Cartwright
Input file : "C:\Users\shau7031\AppData\Roaming\geneGIS\spagedi_data.txt"
Results file : "Z:\data\arcgis\addins\genegis\tests\data\tmxr.txt"
1318 individuals
2 categories: CA-OR, Cent_Am
Latitude-longitude coordinates (assumed to be in degrees; used to compute distances in km), Latitude, Longitude
14 loci: L_Ev1_1, L_Ev1_2, L_Ev104_1, L_Ev104_2, L_Ev14_1, L_Ev14_2, L_Ev21_1, L_Ev21_2, L_Ev37_1, L_Ev37_2, L_Ev94_1, L_Ev94_2, L_Ev96_1, L_Ev96_2
3 digits per allele
Max ploidy announced: 2
# indiv within each ploidy: 2x=165
WARNING: There is(are) 1153 individual(s) without genotype at any locus
# groups (min - max # indiv per group):
2 categorical groups (184 - 1134)
859 spatial groups (1 - 8) WARNING: 543 groups with 1 indiv
859 spatio-categorical groups (1 - 8) WARNING: 543 groups with 1 indiv
GENE DIVERSITY and ALLELE FREQUENCIES
Locus Population Sample size # missing genotypes (%) # incomplete genotypes (%) # of defined gene copies NA: # alleles NAe: Effective # alleles (Nielsen et al. 2003) AR(k=62): Allelic richness (expected number of alleles among 62 gene copies) He (gene diversity corrected for sample size, Nei 1978) Fi (individual inbreeding coefficient) h (gene diversity with UNORDERED alleles, Pons & Petit 1996)
Multilocus average
Multilocus average All populations confounded 1318 1180.1 (89.5%) 0.0 (0.0%) 275.7 7.93 4.04 6.73 0.6783 0.002 0.6741
L_Ev1_1 L_Ev1_1 alleles: 123 125 127 129
L_Ev1_1 All populations confounded 1318 1243 (94.3%) 0 (0.0%) 150 4 2.09 3.07 0.5225 0.159 0.5224 frequency 0.473333 0.00666667 0.506667 0.0133333
L_Ev1_2 L_Ev1_2 alleles: 143 147 149 151 153
L_Ev1_2 All populations confounded 1318 1244 (94.4%) 0 (0.0%) 148 5 1.47 4.07 0.3189 0.068 0.3205 frequency 0.00675676 0.0540541 0.817568 0.108108 0.0135135
L_Ev104_1 L_Ev104_1 alleles: 131 133 135 137 139 141
L_Ev104_1 All populations confounded 1318 1153 (87.5%) 0 (0.0%) 330 6 2.48 5.22 0.5971 -0.056 0.5831 frequency 0.59697 0.163636 0.030303 0.1 0.10303 0.00606061
L_Ev104_2 L_Ev104_2 alleles: 109 111 113 115 117 119
L_Ev104_2 All populations confounded 1318 1245 (94.5%) 0 (0.0%) 146 6 3.44 5.41 0.7097 -0.062 0.7079 frequency 0.109589 0.417808 0.0547945 0.308219 0.10274 0.00684932
L_Ev14_1 L_Ev14_1 alleles: 192 194 196 198 200 202 204 208 210 212 214 216 220
L_Ev14_1 All populations confounded 1318 1155 (87.6%) 0 (0.0%) 326 13 8.39 11.02 0.8809 -0.003 0.8824 frequency 0.0889571 0.00306748 0.0644172 0.202454 0.101227 0.122699 0.0214724 0.0981595 0.165644 0.0521472 0.0521472 0.00613497 0.0214724
L_Ev14_2 L_Ev14_2 alleles: 206 208 212 214 216 218
L_Ev14_2 All populations confounded 1318 1254 (95.1%) 0 (0.0%) 128 6 3.81 5.47 0.7375 0.090 0.7324 frequency 0.101563 0.046875 0.351563 0.320313 0.171875 0.0078125
L_Ev21_1 L_Ev21_1 alleles: 143 147 149 157 159 161 163 165 167 169
L_Ev21_1 All populations confounded 1318 1154 (87.6%) 0 (0.0%) 328 10 5.11 8.69 0.8044 -0.001 0.7978 frequency 0.0152439 0.0152439 0.0579268 0.112805 0.109756 0.192073 0.347561 0.10061 0.0396341 0.00914634
L_Ev21_2 L_Ev21_2 alleles: 147 155 159 175 179 183 187
L_Ev21_2 All populations confounded 1318 1153 (87.5%) 0 (0.0%) 330 7 1.36 5.06 0.2648 0.039 0.2615 frequency 0.854545 0.0636364 0.0151515 0.0030303 0.0242424 0.0121212 0.0272727
L_Ev37_1 L_Ev37_1 alleles: 183 195 199 203 206 207 210 211 214 218 222 226 230
L_Ev37_1 All populations confounded 1318 1153 (87.5%) 0 (0.0%) 330 13 6.18 10.47 0.8382 -0.041 0.8275 frequency 0.0030303 0.0848485 0.0363636 0.0636364 0.0151515 0.112121 0.0424242 0.00606061 0.318182 0.127273 0.130303 0.0515152 0.00909091
L_Ev37_2 L_Ev37_2 alleles: 100 104 106 108 110 112 114 116 118
L_Ev37_2 All populations confounded 1318 1153 (87.5%) 0 (0.0%) 330 9 5.28 7.57 0.8104 0.013 0.8060 frequency 0.00909091 0.0515152 0.227273 0.124242 0.184848 0.1 0.0121212 0.278788 0.0121212
L_Ev94_1 L_Ev94_1 alleles: 109 111 113 115 117 119 121
L_Ev94_1 All populations confounded 1318 1155 (87.6%) 0 (0.0%) 326 7 3.39 6.31 0.7054 0.035 0.6960 frequency 0.0552147 0.159509 0.128834 0.48773 0.0644172 0.0981595 0.00613497
L_Ev94_2 L_Ev94_2 alleles: 137 143 145 147 149 151 153 155 157 159 161 163
L_Ev94_2 All populations confounded 1318 1154 (87.6%) 0 (0.0%) 328 12 5.99 9.92 0.8330 0.005 0.8318 frequency 0.0213415 0.0365854 0.0609756 0.0213415 0.17378 0.234756 0.234756 0.0335366 0.0152439 0.0182927 0.00304878 0.146341
L_Ev96_1 L_Ev96_1 alleles: 194 196 198 200 202 204 206
L_Ev96_1 All populations confounded 1318 1153 (87.5%) 0 (0.0%) 330 7 3.93 6.39 0.7456 -0.008 0.7604 frequency 0.0212121 0.366667 0.0909091 0.0151515 0.0515152 0.287879 0.166667
L_Ev96_2 L_Ev96_2 alleles: 112 114 116 118 120 122
L_Ev96_2 All populations confounded 1318 1153 (87.5%) 0 (0.0%) 330 6 3.67 5.50 0.7278 -0.116 0.7082 frequency 0.193939 0.0393939 0.424242 0.148485 0.181818 0.0121212
VALUES OF THE COMPUTED STATISTICS
Genetic analyses at POPULATION level (1 pop = 1 CATEGORICAL GROUP -> 2 populations)
Dist classes 1
Max distance 4535.29
Number of pairs 1
% partic 100.0
CV partic 0.00
Mean distance 4535.29
Mean ln(distance) 8.41964
Global F-statistics Pairwise Fst (ANOVA approach) distance range for regression analyses
Locus Fit Fis Fst 1 0-4535.29 b-lin(slope linear dist) b-log(slope log dist)
ALL LOCI 0.0007 0.0024 -0.0016 -0.0016
L_Ev1_1 0.1588 0.1588 -0.0000 -0.0000
L_Ev1_2 0.0649 0.0715 -0.0070 -0.0070
L_Ev104_1 -0.0571 -0.0551 -0.0019 -0.0019
L_Ev104_2 -0.0658 -0.0583 -0.0071 -0.0071
L_Ev14_1 -0.0008 -0.0042 0.0034 0.0034
L_Ev14_2 0.0833 0.0959 -0.0140 -0.0140
L_Ev21_1 -0.0002 -0.0009 0.0007 0.0007
L_Ev21_2 0.0357 0.0406 -0.0051 -0.0051
L_Ev37_1 -0.0430 -0.0404 -0.0025 -0.0025
L_Ev37_2 0.0116 0.0137 -0.0021 -0.0021
L_Ev94_1 0.0305 0.0370 -0.0067 -0.0067
L_Ev94_2 0.0026 0.0056 -0.0031 -0.0031
L_Ev96_1 -0.0089 -0.0075 -0.0014 -0.0014
L_Ev96_2 -0.1036 -0.1236 0.0178 0.0178
Jackknifed estimators (over loci)
Mean 0.0004 0.0020 -0.0016 -0.0016
SE 0.0163 0.0174 0.0020 0.0020
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