Created
May 10, 2018 16:19
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Convert Phyloseq to AmpVis2 format in R
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```{r phyloseq-ampvis2} | |
library("ampvis2") | |
# Load data into ampvis | |
otu.table <- t(otu_table(ps1, taxa_are_rows = FALSE)) | |
otu.table <- as.data.frame(otu.table) | |
otu.table <- rownames_to_column(otu.table, var = "OTU") | |
head(rownames(otu.table)) | |
head(colnames(otu.table)) | |
nrow(otu.table) | |
tax.table <- as.data.frame(tax_table(ps1)) | |
tax.table <- rownames_to_column(tax.table, var = "OTU") | |
head(rownames(tax.table)) | |
head(colnames(tax.table)) | |
nrow(tax.table) | |
my_otu_table <- left_join(otu.table, tax.table, by = "OTU") | |
nrow(my_otu_table) | |
colnames(my_otu_table) | |
head(rownames(my_otu_table)) | |
my_otu_table <- column_to_rownames(my_otu_table, var = "OTU") | |
head(rownames(my_otu_table)) | |
colnames(my_otu_table) | |
nrow(my_otu_table) | |
my_metadata <- as.tibble(sample_data(ps1)) | |
class(my_metadata) | |
head(rownames(my_metadata)) | |
nrow(my_metadata) | |
ncol(my_metadata) | |
my_tree <- phy_tree(ps1) | |
ps1.av2 <- amp_load(otutable = my_otu_table, metadata = my_metadata, tree = my_tree) | |
ps1.av2 | |
# Heatmap | |
amp_heatmap(ps1.av2, group_by = "day", facet_by = "randomization_arm") | |
# Boxplot | |
amp_boxplot(ps1.av2, group_by = "day", tax_add = "Phylum") | |
``` |
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