This is an example of combining a folder of songs into one mp3 file using pydub (http://pydub.com/).

pydub-combine-songs

import os
from glob import glob
from pydub import AudioSegment
AudioSegment.converter = '/usr/local/bin/ffmpeg'

playlist_songs = [AudioSegment.from_mp3(file) for file in glob('songs/*.mp3')]
combined = AudioSegment.empty()

for song in playlist_songs:
  combined += song

combined.export('combined.mp3', format='mp3')