main.rb

#!/usr/bin/env ruby

# Write loaded ruby file
File.write('./loaded_file.rb', 'puts :hello')

# load file and write iseq file
iseq = RubyVM::InstructionSequence.compile_file('./loaded_file.rb')
File.write('./loaded_file.yarb', iseq.to_binary)

# define load_iseq
class RubyVM::InstructionSequence
  def self.load_iseq(fname)
    b = File.read('./loaded_file.yarb')
    RubyVM::InstructionSequence.load_from_binary(b)
  end
end

TracePoint.trace(:line) do |tp|
  puts "file: #{tp.path}, line: #{tp.lineno}" if tp.path =~ /loaded_file/
end

load './loaded_file.rb'