connection_routes_all_routes.py

with open('connection_lines.geojson') as connection_lines_file:
    connection_lines = json.loads(connection_lines_file.read())

route_features = []
for line_feature in connection_lines['features']:

    origin = {
        'type': 'Feature',
        'geometry': {
            'type': 'Point',
            'coordinates': line_feature['geometry']['coordinates'][0]}}
    destination = {
        'type': 'Feature',
        'geometry': {
                'type': 'Point',
                'coordinates': line_feature['geometry']['coordinates'][1]}}

    directions_service = Directions(
        access_token=os.environ['MAPBOX_ACCESS_TOKEN'])

    response = directions_service.directions(
        [origin, destination], 'mapbox.driving', alternatives=False)

    route_feature = response.geojson()['features'][0]
    route_feature['properties'] = line_feature['properties']

    route_features.append(route_feature)

routes_gdf = gpd.GeoDataFrame.from_features(route_features)