Convert a number of individual frames to a compressed MP4 movie with ffmpeg on Linux from Python

run ffmpeg for pTIRF movie.py

# https://chat.openai.com/c/cff8e0fc-20df-4425-9e2b-7aa8228ab7a1

## Install ffmpeg on RHEL8: https://www.benholcomb.com/ffmpeg-on-rhel8/
## After that
# sudo yum install python3-devel

## Create virtual environment
# python3 -m venv path/to/venv
# cd path/to/venv
# bin/activate
## install imageio and ffmpeg wrapper
# pip install imageio[ffmpeg]

import os
import imageio

input_folder = '/research_jude/common/Roman/frames/'
output_file = '/research_jude/common/Roman/ffmpeg_output.mp4'

# List all image files in the input folder
image_files = [f for f in os.listdir(input_folder) if f.endswith('.tif')]
image_files.sort()  # Ensure the frames are in the correct order

# Create a writer for the output video file with custom FFmpeg options
writer = imageio.get_writer(output_file, codec='libx264', ffmpeg_params=['-crf', '30'])

# Iterate through image files and write to the output file
for image_file in image_files:
    image_path = os.path.join(input_folder, image_file)
    frame = imageio.imread(image_path)
    writer.append_data(frame)

# Close the writer to finalize the output file
writer.close()