__main__.py

import time
import pygame

pygame.init()
size = (700, 500)
screen = pygame.display.set_mode(size)

pygame.display.set_caption("444ms click")
done = False
clock = pygame.time.Clock()
# from http://soundbible.com/184-Button-Click-On.html
click = pygame.mixer.Sound("Button Click On-SoundBible.com-459633989.wav").play
TICK = pygame.USEREVENT + 1
pygame.time.set_timer(TICK, 440)
timer = pygame.time.get_ticks
start = now = timer()
while not done:
    for event in pygame.event.get():
        if event.type == pygame.QUIT:
            done = True
        if event.type == TICK:
            now = timer()
            print(now - start)
            start = now
            click()

    clock.tick(60)

pygame.quit()