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@zkamvar
Last active Aug 29, 2015
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A set of functions to detect potential issues with repeat length specifications for use with Bruvo's distance in poppr
#' Test repeat length consistency.
#'
#' This function will test for consistency in the sense that all alleles are
#' able to be represented as discrete units after division and rounding.
#' @param gid a genind object
#' @param replen a numeric vector of repeat motif lengths.
#' @return a logical vector indicating whether or not the repeat motif length is
#' consistent.
#'
#' @details This function is modified from the version used in
#' \url{http://dx.doi.org/10.5281/zenodo.13007}.
#'
#' @references Zhian N. Kamvar, Meg M. Larsen, Alan M. Kanaskie, Everett M.
#' Hansen, & Niklaus J. Grünwald. Sudden_Oak_Death_in_Oregon_Forests: Spatial
#' and temporal population dynamics of the sudden oak death epidemic in Oregon
#' Forests. ZENODO, http://doi.org/10.5281/zenodo.13007, 2014.
#'
#' Ruzica Bruvo, Nicolaas K. Michiels, Thomas G. D'Souza, and Hinrich
#' Schulenburg. A simple method for the calculation of microsatellite genotype
#' distances irrespective of ploidy level. Molecular Ecology, 13(7):2101-2106,
#' 2004.
#'
#' @export
#' @author Zhian N. Kamvar
#' @examples
#' data(nancycats)
#' test_replen(nancycats, rep(2, 9))
test_replen <- function(gid, replen){
alleles <- lapply(gid@all.names, as.numeric)
are_consistent <- vapply(1:nLoc(gid), consistent_replen, logical(1),
alleles, replen)
names(are_consistent) <- locNames(gid)
return(are_consistent)
}
consistent_replen <- function(index, alleles, replen){
!any(duplicated(round(alleles[[index]]/replen[index])))
}
#' Find and fix inconsistent repeat lengths
#'
#' Attempts to fix inconsistent repeat lengths found by \code{test_replen}
#'
#' @param gid a genind object
#' @param replen a numeric vector of repeat motif lengths.
#' @param e a number to be subtracted or added to inconsistent repeat lengths to
#' allow for proper rounding.
#' @param fix_some if \code{TRUE} (default), when there are inconsistent repeat
#' lengths that cannot be fixed by subtracting or adding e, those than can be
#' fixed will. If \code{FALSE}, the original repeat lengths will not be fixed.
#' @return a numeric vector of corrected reapeat motif lengths.
#' @details This function is modified from the version used in
#' \url{http://dx.doi.org/10.5281/zenodo.13007}.\cr Before being fed into the
#' algorithm to calculate Bruvo's distance, the amplicon length is divided by
#' the repeat unit length. Because of the amplified primer sequence attached
#' to sequence repeat, this division does not always result in an integer and
#' so the resulting numbers are rounded. The rounding also protects against
#' slight mis-calls of alleles. Because we know that \deqn{\frac{(A - e) - (B
#' - e)}{r}}{((A - e) - (B - e))/r} is equivalent to \deqn{\frac{A - B}{r}}{(A
#' - B)/r}, we know that the primer sequence will not alter the relationships
#' between the alleles. Unfortunately for nucleotide repeats that have powers
#' of 2, rounding in R is based off of the IEC 60559 standard (see
#' \code{\link{round}}), that means that any number ending in 5 is rounded to
#' the nearest \emph{even} digit. This function will attempt to alleviate this
#' problem by adding a very small amount to the repeat length so that division
#' will not result in a 0.5. If this fails, the same amount will be
#' subtracted. If neither of these work, a warning will be issued and it is up
#' to the user to determine if the fault is in the allele calls or the repeat
#' lengths.
#' @export
#'
#' @references Zhian N. Kamvar, Meg M. Larsen, Alan M. Kanaskie, Everett M.
#' Hansen, & Niklaus J. Grünwald. Sudden_Oak_Death_in_Oregon_Forests: Spatial
#' and temporal population dynamics of the sudden oak death epidemic in Oregon
#' Forests. ZENODO, http://doi.org/10.5281/zenodo.13007, 2014.
#'
#' Ruzica Bruvo, Nicolaas K. Michiels, Thomas G. D'Souza, and Hinrich
#' Schulenburg. A simple method for the calculation of microsatellite genotype
#' distances irrespective of ploidy level. Molecular Ecology, 13(7):2101-2106,
#' 2004.
#'
#' @author Zhian N. Kamvar
#' @examples
#'
#' data(nancycats)
#' fix_replen(nancycats, rep(2, 9))
#' # Let's start with an example of a tetranucleotide repeat motif and imagine
#' # that there are twenty alleles all 1 step apart:
#' (x <- 1:20L * 4L)
#' # These are the true lengths of the different alleles. Now, let's add the
#' # primer sequence to them.
#' (PxP <- x + 21 + 21)
#' # Now we make sure that x / 4 is equal to 1:20, which we know each have
#' # 1 difference.
#' x/4
#' # Now, we divide the sequence with the primers by 4 and see what happens.
#' (PxPc <- PxP/4)
#' (PxPcr <- round(PxPc))
#' diff(PxPcr) # we expect all 1s
#'
#' # Let's try that again by subtracting a tiny amount from 4
#' (PxPc <- PxP/(4 - 1e-5))
#' (PxPcr <- round(PxPc))
#' diff(PxPcr)
fix_replen <- function(gid, replen, e = 1e-5, fix_some = TRUE){
if (length(replen) != nLoc(gid)) {
stop(paste0("length of repeats (", length(replen), ") does not equal",
" the number of loci (", nLoc(gid), ")."))
}
consistent_reps <- test_replen(gid, replen)
names(replen) <- locNames(gid)
ADD <- FALSE
SUB <- FALSE
newReps <- replen
while (any(!consistent_reps)){
if (!SUB){
newReps[!consistent_reps] <- newReps[!consistent_reps] - e
SUB <- TRUE
} else {
newReps[!consistent_reps] <- newReps[!consistent_reps] + (2*e)
ADD <- TRUE
}
consistent_reps <- test_replen(gid, newReps)
if (any(!consistent_reps) & ADD & SUB){
inconsistent <- paste(names(replen[!consistent_reps]), collapse = ", ")
msg <- paste("The repeat lengths for", inconsistent,
"are not consistent.\n\n",
"This might be due to inconsistent allele calls or repeat",
"lengths that are too large.\n",
"Check the alleles to make sure there are no duplicated",
"or similar alleles that might end up being the same after",
"division.\n")
if (fix_some){
original <- test_replen(gid, replen)
fixed <- paste(names(replen[!original & consistent_reps]), collapse = ", ")
msg <- paste(msg, "\nRepeat lengths with some modification are",
"being returned:", fixed)
newReps[!consistent_reps] <- replen[!consistent_reps]
} else {
msg <- paste(msg, "\nOriginal repeat lengths are being returned.")
newReps <- replen
}
warning(msg, immediate. = TRUE)
consistent_reps <- TRUE
}
}
return(newReps)
}
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zkamvar commented Feb 27, 2015

Installation:

devtools::source_gist("b8408ed40924d9d1adb9")

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