Zewen Kelvin Tuong zktuong
eeelinn
/ Batch Counting IHC.ijm
Last active
September 30, 2017 18:50
ImageJ macro that semi-automatically counts multiple DAB positively stained nuclei samples from IHC experiments in a batch. Influenced by: https://gist.github.com/zktuong/2b281aed13a089abb1de2b03a177754d
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| //Title: Batch Counting IHC | |
| //Ver. 1 ORS_20170828 | |
| //Author: Erikka Linn | |
| //NOTE: Before counting, please go to Analyze>>Set Scale and set the scale for image in pixels/um. Check "global" to maintain the same scale for all the images. | |
| input = getDirectory("Input directory"); //Folder with images you want counted | |
| output = getDirectory("Output directory"); //Folder where you want to save the new data (saves image of positive and total cells each) | |
| keyword = "5HT"; //Keyword in the file names that you want (in case there are other photos in the folder) | |
| suffix = ".tif"; //Works with tiff images |