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BjornFJohansson/pAG32.gb

Last active July 29, 2022 16:36
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Properly formatted Genbank sequence for the pAG32 vector
Raw
pAG32.gb
LOCUS pAG32 4160 bp DNA circular SYN 26-NOV-2012
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE Unknown
ORGANISM Unknown Unclassified. . . .
.
REFERENCE 1 (bases 1 to 4160)
AUTHORS Self
JOURNAL Unpublished.
COMMENT Annotated with pLannotate v1.2.0.
ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_feature 91..458
/product="TEF-PRO"
/gene="TEF-PRO"
/label="TEF-PRO"
/ApEinfo_fwdcolor="#00ff69"
/ApEinfo_revcolor="green"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0"
CDS 459..1487
/product="hygromycin B phosphotransferase"
/gene="hph"
/label="hygromycin B phosphotransferase"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0"
misc_feature 1488..1727
/product="TEF-TER"
/gene="TEF-TER"
/label="TEF-TER"
/ApEinfo_fwdcolor="#00ff69"
/ApEinfo_revcolor="green"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0"
CDS complement(2833..3693)
/product="AmpR"
/gene="AmpR"
/label="AmpR"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0"
gene 115..1690
/note="pLannotate"
/label="hphMX6"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="gene"
promoter 115..458
/note="pLannotate"
/label="TEF promoter"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="promoter"
terminator 1493..1690
/note="pLannotate"
/label="TEF terminator"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="terminator"
promoter complement(3694..3798)
/note="pLannotate"
/label="AmpR promoter"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="promoter"
CDS 465..1486
/note="pLannotate"
/label="HygR (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="99.6"
/fragment="True"
/other="CDS"
CDS complement(2833..3693)
/note="pLannotate"
/label="AmpR"
/database="snapgene"
/identity="99.8"
/match_length="100.0"
/fragment="False"
/other="CDS"
rep_origin complement(2074..2662)
/note="pLannotate"
/label="ori"
/database="snapgene"
/identity="99.7"
/match_length="100.0"
/fragment="False"
/other="rep_origin"
promoter join(4144..4160,1..2)
/note="pLannotate"
/label="SP6 promoter"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="promoter"
promoter complement(1798..1816)
/note="pLannotate"
/label="T7 promoter"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="promoter"
ncRNA 2517..2621
/note="pLannotate"
/label="RNAI"
/database="Rfam"
/identity="100.0"
/match_length="102.9"
/fragment="False"
/other="ncRNA"
misc_feature 4030..4081
/note="pLannotate"
/label="bom (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="36.9"
/fragment="True"
/other="misc_feature"
CDS 3893..3928
/note="pLannotate"
/label="rop (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="18.8"
/fragment="True"
/other="CDS"
intron complement(1793..1819)
/note="pLannotate"
/label="CMV intron (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="24.5"
/fragment="True"
/other="intron"
CDS complement(1838..1891)
/note="pLannotate"
/label="lacI (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="5.0"
/fragment="True"
/other="CDS"
CDS 82..171
/note="pLannotate"
/label="MRL1 (fragment)"
/database="swissprot"
/identity="96.7"
/match_length="11.0"
/fragment="True"
/other="CDS"
ORIGIN
1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
61 aggcgcgcca gatctgttta gcttgccttg tccccgccgg gtcacccggc cagcgacatg
121 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
181 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
241 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
301 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
361 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
421 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaaaag cctgaactca
481 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
541 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
601 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
661 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
721 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
781 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
841 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
901 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
961 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
1021 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
1081 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
1141 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
1201 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
1261 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
1321 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
1381 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
1441 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatca gtactgacaa
1501 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt
1561 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc
1621 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt
1681 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctcgaa
1741 ttcatcgatg atatcagatc cactagtggc ctatgcggcc gcggatctgc cggtctccct
1801 atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg
1861 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
1921 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
1981 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
2041 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
2101 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
2161 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
2221 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
2281 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
2341 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
2401 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
2461 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
2521 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
2581 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
2641 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
2701 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
2761 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
2821 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
2881 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
2941 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
3001 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
3061 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
3121 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
3181 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
3241 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
3301 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
3361 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
3421 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
3481 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
3541 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
3601 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
3661 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
3721 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
3781 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
3841 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
3901 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
3961 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
4021 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
4081 ggacatattg tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
4141 acgatttagg tgacactata
//
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