aspera installation manual download page
wget https://download.asperasoft.com/download/sw/cli/3.7.7/aspera-cli-3.7.7.608.927cce8-linux-64-release.sh
update permissions of the installation executable and execute
chmod a+x ./aspera-cli-3.7.7.608.927cce8-linux-64-release.sh
./aspera-cli-3.7.7.608.927cce8-linux-64-release.sh
| #' Convert counts to transcripts per million (TPM). | |
| #' | |
| #' Convert a numeric matrix of features (rows) and conditions (columns) with | |
| #' raw feature counts to transcripts per million. | |
| #' | |
| #' Lior Pachter. Models for transcript quantification from RNA-Seq. | |
| #' arXiv:1104.3889v2 | |
| #' | |
| #' Wagner, et al. Measurement of mRNA abundance using RNA-seq data: | |
| #' RPKM measure is inconsistent among samples. Theory Biosci. 24 July 2012. |
| # install | |
| source("http://bioconductor.org/biocLite.R") | |
| biocLite("flowCore") | |
| # convert | |
| library(tools) # for file_path_sans_ext | |
| library(flowCore) | |
| in.fname <- 'flow123.LMD' | |
| folder <- 'C:\\users\\user\\flow\\' |