Created
April 27, 2018 08:40
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bowtie test
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$ docker run --rm -v $(pwd)/data2:/data comics/bowtie2 wgsim -e 0 -N 10000 -1 300 -2 300 -r 0 -R 0 /data/s_cerevisiae.fa /data/sample_seq_1.fastq /data/sample_seq_2.fastq | |
[wgsim] seed = 1524818001 | |
[wgsim_core] calculating the total length of the reference sequence... | |
[wgsim_core] 18 sequences, total length: 12162995 | |
$ ls -alh data2 | |
total 25M | |
drwxr-xr-x 2 alper alper 4.0K Apr 27 11:32 . | |
drwxr-xr-x 4 alper alper 4.0K Apr 27 11:31 .. | |
-rw-r--r-- 1 root root 6.2M Apr 27 11:33 sample_seq_1.fastq | |
-rw-r--r-- 1 root root 6.2M Apr 27 11:33 sample_seq_2.fastq | |
-rw-r--r-- 1 alper alper 12M Apr 27 11:31 s_cerevisiae.fa | |
$ head GCF_000001405.38_GRCh38.p12_genomic.fna | |
>NC_000001.11 Homo sapiens chromosome 1, GRCh38.p12 Primary Assembly | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN |
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docker run --memory 5g --rm -v A:\Homosapiens:/data alperyilmaz/bowtie2-samtools bowtie2 -
x hg18 -1 h.sapiens_seq_1.fastq -2 h.sapiens_seq_2.fastq -S output1.sam
Killed
(ERR): bowtie2-align exited with value 137