Created
May 12, 2020 15:39
-
-
Save mr-eyes/a21f32cf5f622bb21c63f25178e7b688 to your computer and use it in GitHub Desktop.
Kseq Paired End Fastx reader in chunks
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
// to compile: gcc this_prog.c -lz | |
#include <zlib.h> | |
#include <cstdio> | |
#include "kseq.h" | |
#include <iostream> | |
#include <vector> | |
KSEQ_INIT(gzFile, gzread) | |
using namespace std; | |
class kseqReader { | |
private: | |
bool END = false; | |
string R1_fastx, R2_fastx; | |
int chunk_size; | |
gzFile fp_r1, fp_r2; | |
kseq_t *seq_1, *seq_2; | |
public: | |
struct peRead { | |
string R1_seq; | |
string R2_seq; | |
string R1_name; | |
string R2_name; | |
}; | |
std::vector<peRead> chunk_reads; | |
kseqReader(string R1File, string R2File, int chunk_size) { | |
this->chunk_size = chunk_size; | |
this->R1_fastx = R1File; | |
this->R2_fastx = R2File; | |
fp_r1 = gzopen(R1File.c_str(), "r"); | |
fp_r2 = gzopen(R2File.c_str(), "r"); | |
seq_1 = kseq_init(fp_r1); | |
seq_2 = kseq_init(fp_r2); | |
}; | |
std::vector<peRead> *next_chunk() { | |
this->chunk_reads.clear(); | |
for (int i = 0; i < this->chunk_size && ((kseq_read(seq_1)) >= 0 && (kseq_read(seq_2)) >= 0); i++) { | |
this->chunk_reads.emplace_back(peRead{seq_1->seq.s, seq_2->seq.s, seq_1->name.s, seq_2->name.s}); | |
} | |
if ((int) this->chunk_reads.size() != this->chunk_size) { | |
this->END = true; | |
} | |
return &this->chunk_reads; | |
} | |
[[nodiscard]] bool end() const { | |
return this->END; | |
} | |
~kseqReader() { | |
kseq_destroy(this->seq_1); | |
kseq_destroy(this->seq_2); | |
gzclose(this->fp_r1); | |
gzclose(this->fp_r2); | |
} | |
}; | |
int main(int argc, char *argv[]) { | |
if (argc < 4) { | |
fprintf(stderr, "Usage: %s <in_1.fasta> <in_2.fasta> <chunk_size>\n", argv[0]); | |
return 1; | |
} | |
string R1_file = argv[1]; | |
string R2_file = argv[2]; | |
int chunkSize = stoi(argv[3]); | |
auto *PEReader = new kseqReader(R1_file, R2_file, chunkSize); | |
while (!PEReader->end()) { | |
for (auto &PE : *PEReader->next_chunk()) { | |
cout << PE.R1_name << endl; | |
cout << PE.R1_seq << endl; | |
cout << endl; | |
} | |
} | |
delete PEReader; | |
return 0; | |
} |
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment