boto3_DO-spaces.py

from boto3 import session
from botocore.client import Config

ACCESS_ID = 'XXXXXXX'
SECRET_KEY = 'XXXXXXX'

# Initiate session
session = session.Session()
client = session.client('s3',
                        region_name='nyc3',
                        endpoint_url='https://nyc3.digitaloceanspaces.com',
                        aws_access_key_id=ACCESS_ID,
                        aws_secret_access_key=SECRET_KEY)

# Upload a file to your Space
client.upload_file('test.html', 'hello-spaces', 'new-folder/new_file_name.html')

I think last line of the example is incorrect, from docs, this should be:

client.upload_file('new-folder/new_file_name.html', 'hello-spaces', 'test.html')