WSL 2 uses a Hyper-V Virtual Network adapter. Network connectivity works without any issue when a VPN is not in use. However when a Cisco AnyConnect VPN session is established Firewall Rules and Routes are added which breaks connectivity within the WSL 2 VM. This issue is tracked WSL/issues/4277
Below outline steps to automatically configure the Interface metric on VPN connect and update DNS settings (/etc/resolv.conf) on connect/disconnect.
| mkdir -p $PREFIX/bin $PREFIX/lib $PREFIX/share | |
| ls -lha | |
| cp bin/* $PREFIX/bin/ | |
| cp -r lib/* $PREFIX/lib/ | |
| cp -r share/* $PREFIX/share/ |
| channels: | |
| - conda-forge | |
| - bioconda | |
| dependencies: | |
| - python>=3.7 | |
| - samtools=1.10 | |
| - bcftools=1.10 | |
| - pyyaml | |
| - click |
| 20 44564973 44564980 pilot | |
| 20 44564981 44565001 pilot |
| """ | |
| MIT License | |
| Copyright (c) 2020 Warren W. Kretzschmar | |
| Permission is hereby granted, free of charge, to any person obtaining a copy | |
| of this software and associated documentation files (the "Software"), to deal | |
| in the Software without restriction, including without limitation the rights | |
| to use, copy, modify, merge, publish, distribute, sublicense, and/or sell | |
| copies of the Software, and to permit persons to whom the Software is |
| import math | |
| import yaml | |
| import pysam | |
| def main(samfile_name, chrom, start, end): | |
| """ | |
| :param samfile_name: | |
| :param chrom: string |
| rule prepare_out_mat_for_mrbayes: | |
| input: | |
| gt='results/shearwater/Shearwater_calls_FDR0.95_all_muts.gt{gt_num}.txt.gz', | |
| output: | |
| mat='results/mrbayes/shearwater.gt{gt_num, \d+}/Shearwater_calls_FDR0.95_all_muts.gt{gt_num}.mat.gz', | |
| combined='results/mrbayes/shearwater.gt{gt_num, \d+}/Shearwater_calls_FDR0.95_all_muts.gt{gt_num}.sample_mat.txt', | |
| shell: | |
| """ | |
| gzip -dc {input.gt} | cut -f 5-144 | sed 's/NA/?/g' | rs -c'\t' -C'\t' -T | sed 's/\t//g' | gzip -c > {output.mat} | |
| set +o pipefail |
| params.in = "$baseDir/reads.fa" | |
| sequences = file(params.in) | |
| process test { | |
| conda 'transabyss' | |
| input: | |
| file 'input.fa' from sequences | |
| output: | |
| file 'transcripts.fa' into results |
| from zipfile import ZipFile | |
| text = b'hello world' | |
| with ZipFile('spam.zip', 'w') as inzip: | |
| with inzip.open('eggs.txt', 'w') as infile: | |
| infile.write(text) | |
| with ZipFile('spam.zip', 'r') as myzip: | |
| with myzip.open('eggs.txt', 'r') as myfile: |
| >0 | |
| CGTT | |
| >1 | |
| CGTA |