There are 2 files need to update in order to add support for more GPIO pins

ameba_RTL8722DM_add_more_GPIO.cpp

// "variant.cpp"

PinDescription g_APinDescription[TOTAL_GPIO_PIN_NUM]=
{
    {PB_2,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D0
    {PB_1,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D1
    {PB_3,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D2
    {PB_31,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D3
    {PB_30,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D4
    {PB_28,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D5
    {PB_29,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D6
    {NC,       NOT_INITIAL, NOT_INITIAL                          , NOT_INITIAL}, //D7
    {PB_22,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D8
    {PB_23,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D9
    {PB_21,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D10
    {PB_18,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D11
    {PB_19,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D12
    {PB_20,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D13
    {PA_7,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D14
    {PA_8,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D15
    {PA_25,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D16
    {PA_26,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D17
    {PB_7,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D18
    {PB_6,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D19
    {PB_5,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D20
    {PB_4,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D21
    {PA_28,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D22
    {PA_24,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D23
    {PA_23,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D24
    {PA_22,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D25
    {PA_21,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D26
    {PA_20,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D27
    {PA_19,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D28
    {PA_12,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}  //D29  This pin is on the male header!
};

/////////////////////////////////////////////////////////

// "varient.h

#define TOTAL_GPIO_PIN_NUM            (30) // default is 29, you should increment this number with the number of new pins added

It does, please take a closer look at the last line of my gist, you have to increment the macro "TOTAL_GPIO_PIN_NUM " under variant.h before you can add new line.

Also, you shouldn't delete any existing pin declaration from the matrix, you should only add new lines following the end of the last line in the matrix.

why not you try and copy my code and paste to both variant.cpp and variant.h? I have just tested it, and it works for me ;)

Oh, nice.

I didn't saw the last line. 🤦‍♂️
I need to familiarize with the source code at all.

PB[24] = HS_USI_I2C_SCL
PB[25] = HS_USI_I2C_SDA

Can you please help me to enable these I2C port?

Oh, nice.

I didn't saw the last line. 🤦‍♂️
I need to familiarize with the source code at all.

PB[24] = HS_USI_I2C_SCL
PB[25] = HS_USI_I2C_SDA

Can you please help me to enable these I2C port?

Hi, to add new set of I2C, you should look at this line,
https://github.com/ambiot/ambd_arduino/blob/5b62e45d6721404532d12c3c5397033723f92acd/Arduino_package/hardware/libraries/Wire/Wire.cpp#L275

e.g.:
TwoWire Wire2 = TwoWire(PB_25, PB_24);