There are 2 files need to update in order to add support for more GPIO pins

ameba_RTL8722DM_add_more_GPIO.cpp

// "variant.cpp"

PinDescription g_APinDescription[TOTAL_GPIO_PIN_NUM]=
{
    {PB_2,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D0
    {PB_1,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D1
    {PB_3,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D2
    {PB_31,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D3
    {PB_30,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D4
    {PB_28,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D5
    {PB_29,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D6
    {NC,       NOT_INITIAL, NOT_INITIAL                          , NOT_INITIAL}, //D7
    {PB_22,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D8
    {PB_23,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D9
    {PB_21,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D10
    {PB_18,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D11
    {PB_19,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D12
    {PB_20,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D13
    {PA_7,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D14
    {PA_8,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D15
    {PA_25,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D16
    {PA_26,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D17
    {PB_7,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D18
    {PB_6,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D19
    {PB_5,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D20
    {PB_4,     NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D21
    {PA_28,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D22
    {PA_24,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D23
    {PA_23,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ | PIO_PWM    , NOT_INITIAL}, //D24
    {PA_22,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D25
    {PA_21,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D26
    {PA_20,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D27
    {PA_19,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}, //D28
    {PA_12,    NOT_INITIAL, PIO_GPIO | PIO_GPIO_IRQ              , NOT_INITIAL}  //D29  This pin is on the male header!
};

/////////////////////////////////////////////////////////

// "varient.h

#define TOTAL_GPIO_PIN_NUM            (30) // default is 29, you should increment this number with the number of new pins added

Oh, nice.

I didn't saw the last line. 🤦‍♂️
I need to familiarize with the source code at all.

PB[24] = HS_USI_I2C_SCL
PB[25] = HS_USI_I2C_SDA

Can you please help me to enable these I2C port?

Oh, nice.

I didn't saw the last line. 🤦‍♂️
I need to familiarize with the source code at all.

PB[24] = HS_USI_I2C_SCL
PB[25] = HS_USI_I2C_SDA

Can you please help me to enable these I2C port?

Hi, to add new set of I2C, you should look at this line,
https://github.com/ambiot/ambd_arduino/blob/5b62e45d6721404532d12c3c5397033723f92acd/Arduino_package/hardware/libraries/Wire/Wire.cpp#L275

e.g.:
TwoWire Wire2 = TwoWire(PB_25, PB_24);